Chicken Interferon α,IFN-α ELISA Kit
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中文名稱：雞α干擾素(IFN-α)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E10071Ch
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：雞α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E10071Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNA1含量。IFNA1即干擾素α1，是I型干擾素家族成員。它具有抗病毒、抗腫瘤和免疫調(diào)節(jié)等多種作用。其研究機(jī)制主要是通過與細(xì)胞表面受體結(jié)合，激活下游信號(hào)通路，誘導(dǎo)產(chǎn)生多種抗病毒蛋白，抑制病毒復(fù)制和調(diào)節(jié)機(jī)體免疫反應(yīng)。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領(lǐng)域?qū)η蓊惷庖呦到y(tǒng)的機(jī)制研究；支持科研人員在病毒感染模型構(gòu)建、疫苗免疫效果評(píng)估、免疫調(diào)節(jié)劑開發(fā)等研究中動(dòng)態(tài)監(jiān)測天然免疫應(yīng)答強(qiáng)度；為禽類免疫學(xué)研究提供標(biāo)準(zhǔn)化工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：IFNA1; ELISA Kit; IFNA2 ELISA Kit; Interferon type A1/A2 ELISA Kit
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縮寫：IFN-α
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Uniprot No.：
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種屬：Gallus gallus (Chicken)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：3.12 pg/mL-200 pg/mL
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靈敏度：0.78 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 83 Range % 80-95 1:2 Average % 95 Range % 91-101 1:4 Average % 93 Range % 86-98 1:8 Average % 95 Range % 82-98 -
回收率：
The recovery of chicken IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 96 92-103 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 200 1.726 1.745 1.736 1.631 100 1.336 1.327 1.332 1.227 50 0.957 0.976 0.967 0.862 25 0.621 0.631 0.626 0.521 12.5 0.437 0.409 0.423 0.318 6.25 0.303 0.301 0.302 0.197 3.12 0.234 0.244 0.239 0.134 0 0.107 0.102 0.105 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Has antiviral activities.
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亞細(xì)胞定位：Secreted.
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蛋白家族：Alpha/beta interferon family
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數(shù)據(jù)庫鏈接：
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