Chicken Heat Shock Protein 90,HSP-90 ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞熱休克蛋白90(HSP-90)酶聯(lián)免疫試劑盒（CSB-E12873C）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的HSP90AA1含量。HSP90AA1，也稱為熱休克蛋白90α，是一種分子伴侶，參與細胞周期控制和信號轉(zhuǎn)導。其研究機制涉及通過ATP酶活性誘導靶蛋白構(gòu)象變化，激活靶蛋白。HSP90AA1在多種生理和病理過程中發(fā)揮重要作用，如細胞應激、炎癥和癌癥。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品廣泛用于家禽熱應激機制研究、疾病模型建立、環(huán)境應激評估等領(lǐng)域，特別適合實驗室開展家禽生理調(diào)控、抗逆性育種及藥效評價等科研項目，為揭示HSP - 90在生物體應激響應中的分子機制提供可靠工具，所有應用僅限于科學研究用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

HSP90AA1 ELISA Kit; HSPCAHeat shock protein HSP 90-alpha ELISA Kit
縮寫：

HSP90AA1
Uniprot No.：

P11501
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken HSP-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	83-99
1:8	Average %	95
	Range %	89-98

回收率：

The recovery of chicken HSP-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	95	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from Hsp90 which acquires an open conformation for the next cycle.
亞細胞定位：

Cytoplasm. Melanosome.
蛋白家族：

Heat shock protein 90 family
數(shù)據(jù)庫鏈接：

KEGG: gga:423463

STRING: 9031.ENSGALP00000018498

UniGene: Gga.6482