Chicken Cluster of Differentiation 4(CD4) ELISA Kit
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中文名稱：雞CD4分子(CD4)酶聯(lián)免疫試劑盒
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貨號：CSB-E13114C
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規(guī)格：96T/48T
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價格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：雞CD4分子(CD4)酶聯(lián)免疫試劑盒（CSB-E13114C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD4含量。CD4是一種T細(xì)胞受體共受體，位于T細(xì)胞表面，與MHC II類分子結(jié)合，協(xié)助T細(xì)胞識別抗原。CD4在HIV感染中起關(guān)鍵作用，HIV病毒通過結(jié)合CD4進(jìn)入T細(xì)胞，導(dǎo)致CD4+T細(xì)胞數(shù)量減少，免疫功能受損。研究CD4的功能和調(diào)控機(jī)制對于理解HIV感染和開發(fā)抗病毒藥物具有重要意義。試劑盒檢測范圍為3.125 ng/mL-800 ng/mL，適用于科研領(lǐng)域中對雞免疫系統(tǒng)的功能研究，包括感染性疾病模型中的T細(xì)胞應(yīng)答分析、疫苗免疫效果評估以及免疫抑制性疾病機(jī)制探索等基礎(chǔ)研究，為禽類免疫學(xué)實(shí)驗提供可靠的定量檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：
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Uniprot No.：
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種屬：Gallus gallus (Chicken)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：3.125 ng/mL-800 ng/mL
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靈敏度：3.125 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
84
Range %
80-89
1:2
Average %
91
Range %
87-96
1:4
Average %
92
Range %
87-98
1:8
Average %
101
Range %
97-105
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回收率：
The recovery of chicken CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
88-96
EDTA plasma (n=4)
104
101-107
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
800
0.174
0.172
0.173
200
0.291
0.275
0.283
50
0.508
0.546
0.527
12.5
0.813
0.835
0.824
3.125
1.365
1.398
1.382
0
2.105
2.168
2.137
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數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- DETERMINATION OF DOPAMINE AND SEROTONIN WITH IMMUNE STATE CD4 AND CD8 OF THE NEWCASTLE INFECTION IN BROILERS AH Ulaiwi?,Iraqi Journal of Agricultural Sciences,2023
- Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella Huanzhi Zhao, et al,Parasitology Research,2019
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