Canine Parathyroid hormone(PTH) ELISA Kit
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中文名稱：犬甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E17575c
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規(guī)格：96T/48T
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價(jià)格：￥3800/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：犬甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒（CSB-E17575c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PTH含量。甲狀旁腺激素（PTH）是甲狀旁腺主細(xì)胞分泌的肽類激素。其可調(diào)節(jié)鈣、磷代謝，維持血鈣水平穩(wěn)定。在研究機(jī)制上，PTH作用于骨骼、腎臟等靶器官，通過(guò)與受體結(jié)合激活信號(hào)通路，促進(jìn)骨鈣釋放、腎小管對(duì)鈣重吸收及磷排泄等。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于探索犬鈣代謝異常、甲狀旁腺相關(guān)疾病機(jī)制及內(nèi)分泌調(diào)控研究；為科研領(lǐng)域提供高效工具，支持動(dòng)物模型研究、營(yíng)養(yǎng)代謝實(shí)驗(yàn)及疾病機(jī)制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名：PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of canine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 86-102 1:2 Average % 96 Range % 90-103 1:4 Average % 89 Range % 83-102 1:8 Average % 97 Range % 92-105 -
回收率：
The recovery of caninePTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 82-105 EDTA plasma (n=4) 96 90-102 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.704 2.721 2.713 2.592 500 2.335 2.347 2.341 2.220 250 1.976 1.991 1.984 1.863 125 1.493 1.482 1.488 1.367 62.5 0.997 0.969 0.983 0.862 31.2 0.482 0.476 0.479 0.358 15.6 0.373 0.364 0.369 0.248 0 0.122 0.119 0.121 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
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基因功能參考文獻(xiàn)：
- Acute metabolic and respiratory alkalosis markedly decreased PTH values during normocalcemia and delayed the PTH response to hypocalcemia PMID: 12929937
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亞細(xì)胞定位：Secreted.
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蛋白家族：Parathyroid hormone family
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數(shù)據(jù)庫(kù)鏈接：
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