Canine Luteinizing Hormone,LH ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬黃體生成素(LH)酶聯免疫試劑盒（CSB-E06790c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白類促性腺激素。它在生殖系統中至關重要，參與調節女性月經周期和排卵、男性睪酮合成。研究機制主要圍繞其與受體結合，激活下游信號通路，影響性腺功能和生殖內分泌穩態。試劑盒檢測范圍為0.75 mIU/mL-60 mIU/mL，適用于生殖內分泌研究、繁殖障礙機制探索、絕育手術前后激素水平追蹤以及繁育管理中的排卵期監測等科研場景，為動物生殖生物學、獸醫學基礎研究及寵物繁育技術開發提供高效檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LHBLutropin subunit beta ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit; Lutropin beta chain ELISA Kit; Fragment ELISA Kit
縮寫：

LH
Uniprot No.：

P18842
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.75 mIU/mL-60 mIU/mL
靈敏度：

0.94 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	96
	Range %	92-99
1:4	Average %	101
	Range %	98-105
1:8	Average %	83
	Range %	80-87

回收率：

The recovery of canine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	84-92
EDTA plasma (n=4)	98	92-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
亞細胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數據庫鏈接：

KEGG: cfa:403959

STRING: 9615.ENSCAFP00000005719

UniGene: Cfa.3817