Bovine luteinizing hormone (LH) ELISA kit

Bovine luteinizing hormone (LH) ELISA kit

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中文名稱：

牛黃體生成素（LH）酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E12826B
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

牛黃體生成素（LH）酶聯(lián)免疫試劑盒（CSB-E12826B）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LH含量。LH即黃體生成素，是腺垂體分泌的促性腺激素。其主要作用于性腺，在女性中促進(jìn)排卵和黃體形成，分泌孕激素；在男性中刺激睪丸間質(zhì)細(xì)胞分泌雄激素。研究多聚焦其分泌調(diào)控機(jī)制及在生殖內(nèi)分泌疾病診治中的應(yīng)用。試劑盒檢測(cè)范圍為1.25 mIU/mL-100 mIU/mL，廣泛應(yīng)用于動(dòng)物繁殖生理機(jī)制研究、發(fā)情周期監(jiān)測(cè)、生殖激素調(diào)控實(shí)驗(yàn)等科研領(lǐng)域，亦可用于評(píng)估飼料添加劑或環(huán)境因素對(duì)牛群生殖功能的影響，為畜牧養(yǎng)殖優(yōu)化和基礎(chǔ)醫(yī)學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
縮寫：

LH
Uniprot No.：

P04651
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.25 mIU/mL-100 mIU/mL
靈敏度：

1.56 mIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	88
	Range %	83-94
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-95
EDTA plasma (n=4)	87	85-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
100	0.361	0.368	0.365
37.5	0.538	0.567	0.553
18.75	0.781	0.765	0.773
6.25	1.156	1.126	1.141
1.25	1.662	1.698	1.680
0	2.161	2.178	2.170

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Exploring the impact of exogenous Kisspeptin-10 on ovarian follicular development and estrus behaviour in Lakhimi cows of Assam A DATTA,/,2024
Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Epitope-based in silico peptide design yields peptide-directed antibodies that recognize the buffalo luteinizing hormone V Nayan,International Journal of Biological Macromolecules,2021
A study on stress response and fertility parameters in phenotypically thermotolerant and thermosensitive dairy cows during summer heat stress I Nanas,Reprod Domest Anim,2020
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Association among lipopolysaccharide, the transforming growth factor-beta superfamily, follicular growth, and transcription factors in spontaneous bovine ovarian cysts Colakoglu H E, et al,Domestic Animal Endocrinology,2019
Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed-artificial insemination in murrah buffalo (Bubalus bubalis) RM Selvam..et al,REPRODUCTION IN DOMESTIC ANIMALS,2017
Exploration of Luteinizing hormone in murrah buffalo (Bubalus bubalis) urine: Extended surge window opens door for estrus prediction Ramu Muthu Selvam.et al,General and Comparative Endocrinology,2016

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功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數(shù)據(jù)庫鏈接：

KEGG: bta:280839

STRING: 9913.ENSBTAP00000048635

UniGene: Bt.12926

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.