Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Bovine lipopolysaccharide binding protein (LBP) ELISA kit

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中文名稱：

牛脂多糖結合蛋白(LBP)酶聯免疫試劑盒
貨號：

CSB-E16370B
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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牛脂多糖結合蛋白(LBP)酶聯免疫試劑盒（CSB-E16370B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LBP含量。LBP即脂多糖結合蛋白，它在人體免疫防御中扮演重要角色，能與脂多糖結合。研究機制方面，LBP可增強單核細胞、巨噬細胞等對脂多糖的識別和反應，激活免疫信號通路，在炎癥反應、抗感染等過程發揮作用，對它的研究有助于深入了解免疫機制。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于牛類感染性疾病模型建立、炎癥相關機制研究及LPS介導的病理學分析等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
縮寫：

LBP
Uniprot No.：

Q2TBI0
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-102
1:2	Average %	86
	Range %	82-95
1:4	Average %	98
	Range %	90-107
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	95	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.126	2.150	2.138	2.002
500	1.611	1.623	1.617	1.481
250	1.142	1.158	1.150	1.014
125	0.797	0.780	0.789	0.653
62.5	0.463	0.451	0.457	0.321
31.25	0.340	0.334	0.337	0.201
15.6	0.271	0.275	0.273	0.137
0	0.135	0.136	0.136

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Evaluation of the immune responses against reduced doses of Brucella abortus S19 (calfhood) vaccine in water buffaloes (Bubalus bubalis), India R Shome,Vaccine,2020
Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,Animal Feed Science and Technology,2020

功能：

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
基因功能參考文獻：
1. Our study provides an overview of the gene expression profile between wild LBP and mutant LBP on the LPS-induced inflammatory response of bovine mammary epithelial cells , which will lead to further understanding of the potential effects of LBP mutations on bovine mammary glands. PMID: 26813383
2. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
3. One possible anti-inflammatory mechanism can be attributed to the negative role of BRLBP in suppressing TLR4/NF-kappaB activation mediated by LPS. These findings suggested that BRLBP may be a useful agent to treat LPS-induced mastitis PMID: 25700343
4. Single nucleotide polymorphisms in the lipopolysaccharide-binding protein may hold the secret of susceptibility to clinical mastitis in Chinese Holstein. PMID: 22733492
亞細胞定位：

Secreted. Cytoplasmic granule membrane.
蛋白家族：

BPI/LBP/Plunc superfamily, BPI/LBP family
數據庫鏈接：

KEGG: bta:512242

STRING: 9913.ENSBTAP00000022428

UniGene: Bt.24181

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.