Bovine gonadotropin-releasing hormone(GnRH)ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛促性腺激素釋放激素(GnRH)酶聯免疫試劑盒（CSB-E15857B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GnRH含量。GnRH（促性腺激素釋放激素）是一種由下丘腦分泌的激素，主要調節生殖系統的功能。其研究背景涉及生殖調控、內分泌系統以及腫瘤治療等領域。GnRH通過作用于垂體前葉，調節促性腺激素的分泌，進而影響性腺發育和生殖過程。研究機制主要圍繞GnRH的受體、信號通路及其在不同疾病中的作用展開。試劑盒檢測范圍為100 pg/mL-4000 pg/mL，適用于研究牛生殖周期調控、繁殖力評估、激素分泌動態監測等科研場景，特別適用于畜牧業中生殖內分泌機制探索及生殖健康相關研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

GnRH
Uniprot No.：

Q0VBW7
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

100 pg/mL-4000 pg/mL
靈敏度：

100 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-95
1:2	Average %	95
	Range %	91-101
1:4	Average %	95
	Range %	89-101
1:8	Average %	90
	Range %	85-98

回收率：

The recovery of bovine GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	94-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.