Bovine Vitamin A (VA) ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛維生素A(VA)酶聯(lián)免疫試劑盒（CSB-E17987B）為競(jìng)爭法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VA含量。試劑盒檢測(cè)范圍為6.25 ng/mL-100 ng/mL，靈敏度為4.672 ng/mL。本試劑盒適用于科研場(chǎng)景中牛類樣本的維生素A水平分析，例如探究飼料營養(yǎng)干預(yù)對(duì)VA吸收的影響、疾病狀態(tài)下VA代謝變化或組織分布特征等方向本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

VA
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

4.672 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine VA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	95
	Range %	91-99
1:20	Average %	103
	Range %	99-107
1:40	Average %	98
	Range %	93-103
1:80	Average %	92
	Range %	89-95

回收率：

The recovery of bovine VA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-104
EDTA plasma (n=4)	89	83-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.