Bovine Myoglobin(MB) ELISA kit

說明書下載

產品詳情

產品描述：

牛肌紅蛋白(MB)酶聯免疫試劑盒（CSB-EL013529BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MB含量。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，常用于動物肌肉生理研究、運動醫學模型構建及肉品加工過程中肌肉品質評估等科研領域。適用于探究牛肌肉疾病機制、運動生理學實驗或肌肉相關生物標志物篩選等研究，為畜牧科學、食品科學及基礎醫學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

MB ELISA Kit; Myoglobin ELISA Kit
縮寫：

MB
Uniprot No.：

P02192
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue Homogenates
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	91
	Range %	89-99
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	86-98

回收率：

The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	95	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
基因功能參考文獻：
1. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions. PMID: 26946475
2. Glycine at E14 in myoglobin enhances autoxidation and hemin loss rates. PMID: 22681513
蛋白家族：

Globin family
數據庫鏈接：

KEGG: bta:280695

STRING: 9913.ENSBTAP00000007014

UniGene: Bt.107415