Bovine Interleukin 8,IL-8 ELISA Kit

Bovine Interleukin 8,IL-8 ELISA Kit

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中文名稱：

牛白介素8(IL-8)酶聯免疫試劑盒
貨號：

CSB-E13052B
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

牛白介素8(IL-8)酶聯免疫試劑盒（CSB-E13052B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8 是一種趨化因子，在炎癥反應和免疫調節中起關鍵作用。它能吸引中性粒細胞等免疫細胞到炎癥或損傷部位。在研究機制方面，主要聚焦其與受體結合激活下游信號通路，影響細胞遷移、增殖等，在腫瘤、炎癥疾病治療上有潛在研究價值。試劑盒檢測范圍為50 pg/mL-2000 pg/mL，適用于獸醫學研究、畜牧業動物健康評估及疫苗開發等科研領域，為探索牛類炎癥相關疾病的分子機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
縮寫：

CXCL8
Uniprot No.：

P79255
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

50 pg/mL-2000 pg/mL
靈敏度：

50 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	94
	Range %	86-100
1:4	Average %	88
	Range %	80-94
1:8	Average %	92
	Range %	86-100

回收率：

The recovery of bovine IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-106
EDTA plasma (n=4)	98	94-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.655	2.514	2.585
50	1.279	1.232	1.256
150	0.611	0.638	0.625
460	0.342	0.363	0.353
1000	0.218	0.224	0.221
2000	0.125	0.131	0.128

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Immunological response enhancement in cows with subclinical mastitis fed diet supplemented with Macleaya cordata MSA Khattab, L Haiqiang, T Shaoxun,Frontiers in Veterinary Science,2025
Screening of Some Changes in Hematological, Serum Biochemical, Inflammatory and Oxidative Parameters Associated with Pathogenesis of Retained Placenta in Holstein Dairy Heifers D El-Karim,Journal of Applied Veterinary Sciences,2023
The effect of staphylococcal mastitis including resistant strains on serum procalcitonin, neopterin, acute phase response and stress biomarkers in Holstein dairy cows W El-Deeb,PeerJ,2021
Penta-acetyl Geniposide Suppresses Migration, Invasion, and Inflammation of TNF-α-Stimulated Rheumatoid Arthritis Fibroblast-Like Synoviocytes Involving Wnt/β-Catenin Signaling Pathway L Cai,Inflammation,2021
Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus DF Lee,Scientific reports,2020
Subclinical endometritis and postpartum ovarian resumption in respect to TNF-α, IL-8 and CRP in Egyptian buffaloes DH Elsayed,animal reproduction,2020
Caffeic Acid Prevented LPS-Induced Injury of Primary Bovine Mammary Epithelial Cells through Inhibiting NF-κB and MAPK Activation Mingjiang Liu,Mediators of Inflammation,2019
Supplementation with fat-coated rumen-protected glucose during the transition period enhances milk production and influences blood biochemical parameters of liver function and inflammation in dairy cows X.P.Li,Animal Feed Science and Technology,2019
Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows Ning LT.et al,J Anim Physiol Anim Nutr ,2017
A small scale study on the effects of oral administration of the ß-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves Hirofumi Uchiyama et al,BMC Research Notes,2012

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功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
基因功能參考文獻：
1. Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-kappaB activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1. PMID: 29101849
2. STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. PMID: 26813342
3. As a pilot study, the present results revealed that identified SNPs in IL8 and TLR4 genes can be used as a genetic marker and predisposing factor for resistance/susceptibility to digital dermatitis in dairy cows. However, TLR4 gene may be a potential candidate for such disease. PMID: 27813832
4. Blood levels of cortisol, immunoglobulin G, and IL-8 in relationship to genetics and the incidence of bovine respiratory complex disease are reported. PMID: 27482664
5. Genetic variation at the IL8 locus explains a proportion of the inter-breed and inter-individual variation in immunity between neonatal calves which is likely to influence their resistance to infection. PMID: 27863538
6. In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID: 26342452
7. Studied association of IL8 -105G/A with mastitis somatic cell score in Chinese Holstein dairy cows. Results suggest genotype GG may be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein dairy cows. PMID: 25380467
8. Data implicate a complex cascade of events during luteolysis, involving Il8 signaling, neutrophil recruitment, and immune cell action within the corpus luteum. PMID: 24686456
9. Cadmium exposure induced pulmonary inflammation and increased lung interleukin 8 secretion. PMID: 22094458
10. Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
11. study demonstrates that a small experimental rodent model can be utilized for non-invasively monitoring, through a reporter gene system, the activation of an IL-8 promoter region derived from a larger size animal (bovine) PMID: 22761878
12. Finding of genetic variation in the IL8 promoter that differentially regulates its expression has significant functional implications for IL8 expression in vitro and which may impact on susceptibility to infectious disease and inflammation. PMID: 22244152
13. Polymorphonuclear neutrophils migrate into the early corpus luteum(CL) partially due to its major chemoattractant IL-8 produced at high levels in the CL. PMID: 21651784
14. IL8 genotype significantly correlated with mastitis resistance and the locus could be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein. PMID: 21113675
15. IL8 promoter serial deletion analysis revealed a specific region responsive to ORF50/Rta; may represent an endometrial defense mechanism against viral infection PMID: 20720165
16. IL8 gene genetic diversity has a great genetic effect on milk traits and mastitis resistance PMID: 21513151
17. The role of NF-kappaB and C/EBP factors in regulating basal and pathogen-induced expression of both genes from cattle, is investigated. PMID: 21255844
18. Data describe the expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in bovine theca cells, and suggest that VEGF is associated with the IL system in theca cells in ovary. PMID: 20156197
19. Levels in milk are altered in the presence of bacteria PMID: 15372997
20. C-terminal of both bovine IL-8R showed 100% identity, whereas they differ in most other species, suggesting that the two bovine IL-8R initiate similar signal transduction. PMID: 17727952
21. the pathogen causing subclinical mastitis impairs NF-kappaB activation in MEC thereby severely weakening the immune response,induction of IL-8 and TNFalpha, in the udder PMID: 17936907
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亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數據庫鏈接：

KEGG: bta:280828

STRING: 9913.ENSBTAP00000026275

UniGene: Bt.49740

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.