Bovine Interleukin 12,IL-12 ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛白介素12(IL-12)酶聯免疫試劑盒（CSB-E14010B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12A含量。IL12A為重要靶點，在免疫系統中扮演關鍵角色。其背景上，IL-12由IL12A等亞基組成，可調節免疫反應。研究機制方面，它能促進T細胞和NK細胞的活化、增殖，增強免疫細胞對病原體和腫瘤細胞的殺傷作用，是免疫治療的研究熱點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產品為研究牛類感染性疾病、炎癥反應機制及疫苗免疫效果評估提供可靠工具，尤其適用于畜牧科研中免疫調節機制探索、疾病模型建立等基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
縮寫：

IL12A
Uniprot No.：

P54349
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	98
	Range %	91-102
1:4	Average %	94
	Range %	90-98
1:8	Average %	94
	Range %	86-99

回收率：

The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-92
EDTA plasma (n=4)	97	95-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
基因功能參考文獻：
1. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
2. Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
亞細胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
數據庫鏈接：

KEGG: bta:281856

STRING: 9913.ENSBTAP00000020153

UniGene: Bt.524