Bovine Inhibin beta A chain(INHBA) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛抑制素-βA鏈(INHBA)酶聯免疫試劑盒（CSB-EL011719BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INHBA含量。INHBA是一種靶點。它在細胞生長、分化等生理過程中或發揮重要作用。研究機制上，聚焦于其在信號通路的作用，探索其與疾病發生發展關聯。深入了解它，有助于開發針對該靶點的藥物，為相關疾病治療帶來新思路。試劑盒檢測范圍為15 pg/mL-600 pg/mL，適用于畜牧業中繁殖性能評估、生殖系統疾病模型研究，以及實驗室中對INHBA在組織表達水平、激素調控網絡的相關機制探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

INHBAInhibin beta A chain ELISA Kit; Activin beta-A chain ELISA Kit
縮寫：

INHBA
Uniprot No.：

P07995
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15 pg/mL-600 pg/mL
靈敏度：

15 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-98
1:2	Average %	94
	Range %	86-103
1:4	Average %	92
	Range %	89-98
1:8	Average %	96
	Range %	91-102

回收率：

The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	96	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
基因功能參考文獻：
1. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development. PMID: 27185102
2. The expression of the components of the activin-inhibin-follistatin system is altered in bovine cystic ovarian disease. PMID: 25001504
3. Results support a physiological role for granulosa-derived inhibin as an antagonist of bone morphogenetic protein action on thecal androgen synthesis. PMID: 20739376
4. activin AB is a lignd for activin type IB receptor ALK7 and is responsible for activin-mediated secretion of insulin from pancreatic beta cell line, MIN6. PMID: 15196700
亞細胞定位：

Secreted.
蛋白家族：

TGF-beta family
數據庫鏈接：

KEGG: bta:281867

STRING: 9913.ENSBTAP00000003783

UniGene: Bt.12760