Bovine Caspase 7(Casp-7) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛胱天蛋白酶7(Casp-7)酶聯(lián)免疫試劑盒（CSB-E17315B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Casp7含量。Casp7（Caspase-7）是一種參與細(xì)胞凋亡過(guò)程的半胱氨酸型內(nèi)肽酶，屬于caspase家族。它在細(xì)胞凋亡的執(zhí)行階段發(fā)揮關(guān)鍵作用，通過(guò)切割下游底物如PARP來(lái)促進(jìn)程序性細(xì)胞死亡。Casp7的表達(dá)和活性與多種疾病如癌癥、阿爾茨海默病等密切相關(guān)。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品可用于科研領(lǐng)域細(xì)胞凋亡機(jī)制研究、藥物或毒素誘導(dǎo)的凋亡效應(yīng)評(píng)估、免疫相關(guān)疾病模型分析等場(chǎng)景，例如探究病原感染、腫瘤治療或神經(jīng)系統(tǒng)疾病中的Casp - 7表達(dá)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫：

Casp7
Uniprot No.：

F1MD58
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cell Biology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-101
1:2	Average %	95
	Range %	88-102
1:4	Average %	89
	Range %	83-100
1:8	Average %	93
	Range %	89-106

回收率：

The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-112
EDTA plasma (n=4)	93	84-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.