CRBN
CRBN，別名mental retardation, non-syndromic, autosomal recessive, 2A。CRBN是CRL4CRBN E3泛素連接酶復合物的底物受體，廣泛表達于多種組織中，尤其在神經系統中豐富。CRBN作為底物識別亞單位，通過與DDB1、Cul4A、ROC1形成E3泛素連接酶復合物，促進目標蛋白質的泛素化和隨后的蛋白酶體降解，如MEIS2或ILF2。CRBN維持突觸谷氨酸釋放，對認知功能如記憶和學習至關重要，同時可能通過調節BK通道影響焦慮樣行為。CRBN還在TLR4信號通路中通過與TRAF6和ECSIT相互作用，抑制ECSIT泛素化，從而在信號傳導中起負向調控作用。CRBN的異常功能與多種疾病相關，包括智力發展障礙和某些癌癥，同時也是免疫調節藥物如沙利度胺、來那度胺和泊馬度胺的作用靶點。
熱銷產品
Recombinant Human Protein cereblon (CRBN) (CSB-CF842761HU)
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF842761HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CRBN.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF842761HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CRBN.
CRBN Antibody (CSB-PA842761ESR1HU)
驗證數據
Western Blot
Positive WB detected in: A549 whole cell lysate, U251 whole cell lysate,U87 whole cell lysate,PC-3 whole cell lysate,Hela whole cell lysate,MCF7 whole cell lysate,THP-1 whole cell lysate, Mouse Liver tissue lysate
All lanes: CRBN antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
IHC image of CSB-PA842761ESR1HU diluted at 1:100 and staining in paraffin-embedded human testis tissueperformed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-PA842761ESR1HU diluted at 1:100 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-PA842761ESR1HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
CRBN Antibodies
CRBN for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA005944GA01HU | CRBN Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA842761ESR1HU | CRBN Antibody | Human, Mouse | ELISA, WB, IHC |
| CSB-PA842761ESR2HU | CRBN Antibody | Human, Mouse | ELISA, WB, IHC |
CRBN Proteins
CRBN Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP710067RA CSB-EP710067RA CSB-BP710067RA CSB-MP710067RA CSB-EP710067RA-B |
Recombinant Rat Protein cereblon (Crbn) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF710067RA | Recombinant Rat Protein cereblon (Crbn) | in vitro E.coli expression system |
CRBN Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP806030MO | Recombinant Mouse Protein cereblon (Crbn) | Yeast |
| CSB-EP806030MO CSB-BP806030MO CSB-MP806030MO CSB-EP806030MO-B |
Recombinant Mouse Protein cereblon (Crbn) | E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CRBN Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP842761HU1 CSB-MP842761HU1 |
Recombinant Human Protein cereblon (CRBN), partial | Yeast Mammalian cell |
| CSB-BP842761HU | Recombinant Human Protein cereblon (CRBN) | Baculovirus |
| CSB-BP842761HUc7 | Recombinant Human Protein cereblon (CRBN) | Baculovirus |
| CSB-EP842761HU1 | Recombinant?Human?Protein?cereblon (CRBN), partial | E.coli |
| CSB-EP842761HU1-B | Recombinant Human Protein cereblon (CRBN), partial, Biotinylated | E.coli |
| CSB-MP842761HU | Recombinant Human Protein cereblon (CRBN) | Mammalian cell |
CRBN Proteins for Homo sapiens(Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-CF842761HU | Recombinant Human Protein cereblon (CRBN) | in vitro E.coli expression system |
| CSB-CF842761HUc7 | Recombinant Human Protein cereblon (CRBN) | in vitro E.coli expression system |
| CSB-CF842761HUa6 | Recombinant Human Protein cereblon (CRBN) | in vitro E.coli expression system |
