Recombinant Rat Alpha-crystallin A chain (Cryaa)

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中文名稱：

Recombinant Rat Alpha-crystallin A chain (Cryaa)
貨號：

CSB-EP006007RA
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Cryaa
Uniprot No.：

P24623
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length
來源：

E.coli
分子量：

29.4 kDa
表達區(qū)域：

1-196aa
氨基酸序列

MDVTIQHPWFKRALGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISELMTHMWFVMHQPHAGNPKNNPGKVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Contributes to the transparency and refractive index of the lens. Acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions. Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA.; Inhibits bacterial growth in the lens.
基因功能參考文獻：
1. Rat crystallin alpha A a encode activated chaperones. PMID: 26378715
2. Cryaa is a moonlighting protein that functions as a heat shock protein as well as a lens crystalline. PMID: 7556464
3. The alphaA- and alphaB-crystallin peptides inhibited stress-induced aggregation of four client proteins, and the alphaA-acetyl peptide was more effective than the native peptide against three of the client proteins. PMID: 23508955
4. alphaA-crystallin and alphaB-crystallin reside in separate subcellular compartments in the developing ocular lens PMID: 23071119
5. The main differences, which could be attributed to the cataract-specific processes, are the faster decay of the content of gamma-crystallins and the significant decrease of unmodified alphaA-crystallin abundance in the senescent rat lenses. PMID: 21677790
6. Curcumin suppressed the expression of selenite-induced alphaA- and alphaB-crystallin and Hsp 70, and may therefore suppress cataract formation in rat pups PMID: 21311744
7. hydroxylation and subsequent phosphorylation of a proline residue in alpha-crystallin A in the eye as well as in heart tissue of rat. PMID: 20682783
8. An important role is demonstrated for the C-terminal extension of alpha A crystallin, Arg-163 in particular, whereas no significant role is found for the C-terminal flexible tail in the oligomer assembly of alpha A-crystallin. PMID: 14529298
9. In samples from rats of all ages, the content of alpha A crystallin is significantly decreased in the oldest rats, indicative that protection against stress-induced protein aggregation is compromised in the aged retina. PMID: 14690441
10. The results indicate that elevated expression of alpha-crystallins in some tissues may have implications in pathophysiology of diabetic complications. PMID: 16309625
11. The role of arginine-163 is to provide a positive charge for intersubunit electrostatic interactions in the carboxy-terminal domain of alpha A-crystallin. PMID: 17176090
12. alpha A-K11T and alpha B-K90T/K92T mutants showed the largest decrease in glycation and increase in chaperone activity. PMID: 18158587
13. Enhanced survival of axotomized axons was observed beyond the crush site after a single intravitreal administration of alpha-crystallin at the time of axotomy. PMID: 18551258
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Small heat shock protein (HSP20) family
組織特異性：

Highly expressed in eye lens. Also expressed in non-lenticular tissues such as brain, spleen, liver, lung, skin, small intestine and a several epithelial and fibroblast cell lines with highest levels in spleen.
數(shù)據(jù)庫鏈接：

KEGG: rno:24273

STRING: 10116.ENSRNOP00000065714

UniGene: Rn.127769