tolC Antibody

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說明書

中文名稱：

tolC兔多克隆抗體
貨號：

CSB-PA355904XA01ENV
規格：

￥880
圖片：
Western Blot
Positive WB detected in: recombinant protein
All lanes:tolC Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Escherichia coli (strain K12) tolC Antibody Polyclonal antibody
Uniprot No.：

P02930
基因名：

tolC Antibody
別名：

Outer membrane protein TolC (Multidrug efflux pump subunit TolC) (Outer membrane factor TolC) tolC colE1-i mtcB mukA refI toc weeA b3035 JW5503
宿主：

Rabbit
反應種屬：

Escherichia coli (strain K12)
免疫原：

Recombinant Escherichia coli (strain K12) tolC protein (97-268aa)
免疫原種屬：

Escherichia coli (strain K12)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.
基因功能參考文獻：
1. TolC, a potentially interesting pharmaceutical target can be addressed by small molecules, blocking the channel. Biophysical characterization of the binding processes will support future identification and optimisation of more potent TolC blockers in order to validate TolC as a pharmaceutical target. PMID: 28746830
2. The authors report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that allosterically couples and synchronizes initial ligand binding with channel opening. PMID: 28355133
3. A number of multi-drug efflux genes, particularly the central component TolC, show higher expression in antibiotic resistant Escherichia coli. PMID: 27105118
4. Data suggest that outer membrane channel TolC plays an important role in extrusion of isoprenol and other medium-chain alcohols (CmOHs). PMID: 26563610
5. Thus, tolC mutants grown in low iron conditions accumulate periplasmic enterobactin, which impairs bacterial morphology, possibly by sequestering iron and inhibiting an iron-dependent reaction involved in cell division or peptidoglycan synthesis. PMID: 24330203
6. Interactions between TolC and AcrAB are highly dynamic and only a small fraction of TolC is utilized to support efflux activities. PMID: 23331412
7. The TolC enhancement of extreme-acid survival includes Gad induction, but TolC-dependent growth rates below pH 6.5 do not involve Gad. PMID: 21541325
8. These results indirectly suggest that TolC and YiaT proteins may play some role(s) in multidrug resistance. PMID: 20639525
9. Inactivation of TolC triggers metabolic shutdown in E. coli cells grown in minimal glucose medium. PMID: 20545840
10. essential for enterobactin export across the outer membrane PMID: 16166532
11. The mechanism of tetracycline resistance was studied using expression studies in E. coli mutants that were defective in the tet(A) and the tolC proteins. PMID: 16687460
12. Dada show that a combination of sbmA and tolC mutations appears to have additive effect, resulting in almost complete suppression of the phenotypic expression of Tn10 tetracycline resistance. PMID: 18083810
13. Results describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. PMID: 18406332
14. TolC-dependent efflux system is involved in the exclusion of porphyrins in E. coli. PMID: 18641137
15. The authors conclude that TolC plays an important role in L: -cysteine tolerance probably due to its export ability and that TolC overexpression is effective for L-cysteine production in E. coli. PMID: 18828007
16. Data that TolC is involved in the efflux of certain cellular metabolites that accumulate during growth and trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites. PMID: 19502391
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亞細胞定位：

Cell outer membrane; Multi-pass membrane protein.
蛋白家族：

Outer membrane factor (OMF) (TC 1.B.17) family
數據庫鏈接：

KEGG: ecj:JW5503

STRING: 316385.ECDH10B_3209

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