Human Azurocidin/Heparin Binding Protein(AZU/HBP) ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人天青殺素/肝素結合蛋白(AZU/HBP)酶聯免疫試劑盒（CSB-E09698h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織勻漿、組織培養上清液樣本中的AZU1含量。AZU1，也稱為肝素結合蛋白（HBP）和陽離子抗菌蛋白CAP37，是一種由中性粒細胞顆粒釋放的抗菌和趨化性糖蛋白，屬于肽酶S1家族和彈性酶亞家族。它主要對革蘭氏陰性細菌具有細胞毒性，可能參與炎癥過程中單核細胞的募集。研究顯示，AZU1在急性早幼粒細胞白血病（APL）中表達升高，可能與白血病細胞的分化和治療反應有關。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，支持研究者定量分析AZU/HBP在炎癥反應、免疫調控或感染模型中的動態變化，為探索其作為炎癥相關生物標志物的功能機制提供可靠工具，廣泛應用于免疫學、細胞生物學及疾病機制等科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

AZU1
Uniprot No.：

P20160
種屬：

Homo sapiens (Human)
樣本類型：

serum, urine, tissue homogenates, cell culture supernates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AZU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-91
1:2	Average %	95
	Range %	90-100
1:4	Average %	98
	Range %	92-107
1:8	Average %	92
	Range %	82-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
亞細胞定位：

Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

Peptidase S1 family, Elastase subfamily
數據庫鏈接：

HGNC: 913

OMIM: 162815

KEGG: hsa:566

STRING: 9606.ENSP00000233997

UniGene: Hs.72885